A7549

Anti-Apoptosis-Inducing Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Szinonimák: Anti-AIF
• MDL-szám: MFCD02685955
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ARR; WB
• klón: polyclonal
• faj reaktivitás: mouse, human
• citations: 12
• technika/technikák: microarray: suitable; western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: IgG fraction of antiserum
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 57 kDa
• faj reaktivitás: mouse, human
• kiszerelés: antibody small pack of 25 μL
• technika/technikák: microarray: suitable; western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
• UniProt elérési szám: O95831
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... AIFM1(9131) ; mouse ... Aifm1(26926)

Leírás

Általános leírás

Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).

Apoptosis-inducing factor (AIF) is encoded by the gene mapped to human chromosome X (Xq25-Xq26). It is a 57 kDa mitochondrial flavoprotein. AIF contains two mitochondrial localization sequences and two putative nuclear localization sequences.

Immunogén

synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.

Alkalmazás

Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.

Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.

Biokémiai/fiziológiai hatások

Microinjection of apoptosis-inducing factor (AIF) into the cytoplasm of intact cells induces chromatin condensation, dissipation of the mitochondrial transmembrane potential and exposure of phosphatidylserine in the plasma membrane.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable