Összes fotó(1)
Fontos dokumentumok
A2356
Phosphatase, Alkaline from bovine intestinal mucosa
≥5,500 DEA units/mg protein
Szinonimák:
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
CAS-szám:
MDL-szám:
UNSPSC kód:
12352204
eCl@ss:
42010105
NACRES:
NA.54
Javasolt termékek
Forma
(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
Minőségi szint
specifikus aktivitás
≥5,500 DEA units/mg protein
molekulatömeg
dimer ~160 kDa
koncentráció
≥10 mg/mL
tárolási hőmérséklet
2-8°C
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Alkalmazás
Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Biokémiai/fiziológiai hatások
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.
Fizikai tulajdonságok
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.
Egység definíció
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)
Fizikai forma
Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6
Analízis megjegyzés
Package sizes are based on DEA units
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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Protocols
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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