05066

Agarose

High EEO, for molecular biology

• Szinonimák: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
• CAS-szám: 9012-36-6
• EC-szám: 232-731-8
• MDL-szám: MFCD00081294
• UNSPSC kód: 41105317
• PubChem Substance ID: 24845663
• NACRES: NA.25

Tulajdonságok

• grade: for molecular biology
• Minőségi szint: 100
• Forma: powder
• szennyeződések: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• izzítási maradék: ≤1%
• veszteség: ≤10% loss on drying
• EEO (elektroendozmózis): 0.23-0.27
• átmeneti hőmérséklet: gel point 34-37 °C (1.5% solution)
• gél szilárdság: ≥1500 g/cm²
• anion nyomok: chloride (Cl^-): ≤0.3%; sulfate (SO₄^2-): ≤0.6%
• kation nyomok: Ca: ≤0.05%; Cd: ≤0.001%; Co: ≤0.001%; Cr: ≤0.001%; Cu: ≤0.001%; Fe: ≤0.001%; K: ≤0.05%; Mg: ≤0.001%; Mn: ≤0.001%; Na: ≤0.5%; Ni: ≤0.001%; Pb: ≤0.001%; Zn: ≤0.001%
• SMILES string: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• Nemzetközi kémiai azonosító kulcs: MJQHZNBUODTQTK-WKGBVCLCSA-N

Leírás

Alkalmazás

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Biokémiai/fiziológiai hatások

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Kiszerelés

Packaging
50, 500 g in poly bottle

Analízis megjegyzés

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)