PPB013

1.0% Agarose in Tris-Borate EDTA Buffer

pHast Pack^™, powder

• Szinonimák: 1% Agarose gel, TAE Agarose blend, TBE Agarose blend, TBE buffer garose blend
• UNSPSC kód: 41105317
• NACRES: NA.25

Tulajdonságok

• termékcsalád: pHast Pack^™
• Minőségi szint: 100
• form: powder
• pH: 8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose)
• kation nyomok: Pb: ≤5 ppm
• idegen aktivitás: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; RNase, none detected

Leírás

Általános leírás

1% Agarose in Tris Borate EDTA (TBE buffer, pH 8.3) is a powder blend that dissolves easily in boiling water to quickly make an agarose gel for electrophoresis. Typically, 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp

Alkalmazás

1% Agarose gel in 1X TBE buffer may be used for:

• DNA electrophoresis
• Native RNA Electrophoresis

Tulajdonságok és előnyök

• Ready-to-pour 1% agarose gel eliminates buffer prep
• Accelerates workflow and minimize cost and labor
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm Lead ≤5 ppm
• Compatible with TBE pHast pack^™ buffer

Kiszerelés

Foil pouches

Elkészítési megjegyzés

Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3.

Feloldás

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Tárolás és stabilitás

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Jogi információk

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H360FD
• Óvintézkedésre vonatkozó mondatok: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Veszélyességi osztályok: Repr. 1B
• Tárolási osztály kódja: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1