The information provided by the supplier for SCT117 indicates that the product is at a 200x concentration. To perform staining of fixed and permeabilized cells for cell number normalization, it is recommended to conduct a standard curve of cell density and test RedDot™1 at 1X and 2X final concentration in order to determine the optimal staining concentration for achieving a linear fluorescence signal for your specific cell type.
SCT117
BioTracker NIR694 Nuclear Dye (Water)
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Szinonimák:
Live cell imaging probe
Bejelentkezés a Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
250 μL
139 000,00 Ft
139 000,00 Ft
Available to ship TODAYrészletek
Erről a tételről
UNSPSC kód:
12352207
NACRES:
NA.47
Ugrás ide:
Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?technika/technikák
cell based assay: suitable
észlelési módszer
fluorometric
kiszállítva
ambient
1 of 4
Ez a tétel | SCT119 | SCT118 | SCT120 |
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
Általános leírás
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Alkalmazás
Live cell fluorescent imaging
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. SCT117, BioTracker™ NIR694 Nuclear Dye (Water) is supplied as a 200X stock solution in water
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Fizikai forma
Liquid
Elkészítési megjegyzés
Store BioTracker NIR694 Nuclear Dye (Water) at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analízis megjegyzés
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Absorbance: 662nm
Emission: 694nm
Jogi információk
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Kapcsolódó tartalom
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The data sheet indicates to dilute BioTracker™ NIR694 Nuclear Dye in cell culture medium or PBS to a final concentration of 1X. However, it does not specify the starting concentration, leaving uncertainty about the appropriate dilution ratio. Can you provide guidance on the starting concentration and the amount needed for dilution?
1 answer-
Helpful?
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What is the stock concentration of this product? We purchased it recently, but it doesn’t say on the website or the product documentation.
1 answer-
SCT117 is provided at a 200X concentration.
Helpful?
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