MABS474

Anti-GRP78 Antibody, clone N88

clone N88, from mouse

• Szinonimák: 78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: N88, monoclonal
• application: FACS; WB; activity assay
• faj reaktivitás: human, mouse
• technika/technikák: activity assay: suitable; flow cytometry: suitable; western blot: suitable
• citations: 1

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: N88, monoclonal
• faj reaktivitás: human, mouse
• technika/technikák: activity assay: suitable; flow cytometry: suitable; western blot: suitable
• izotípus: IgG1κ
• NCBI elérési szám: NP_005338
• UniProt elérési szám: P11021
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... HSPA5(3309)

Leírás

Általános leírás

The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER.

Immunogén

Recombinant protein corresponding to human GRP78.

Alkalmazás

Activity Assay Analysis: This antibody accelerates tumor growth in a B16F1 melanoma Flank tumor model (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).
Flow Cytometry Analysis: A representative lot detected GRP78 in B16F1 cells (de Ridder, G. G., et al. (2012). Melanoma Research. 22(3):225-235).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-GRP78 Antibody, clone N88 is validated for use in western blotting, activity assay & flow cytometry for the detection of GRP78.

Minőség

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GRP78 in 10 µg of HeLa cell lysate.

Cél megnevezése

~78 kDa observed

Fizikai forma

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable