MABS1286

Anti-EPO-R Antibody, clone BCO-3H2

clone BCO-3H2, from rat

• Szinonimák: Erythropoietin receptor
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rat
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: BCO-3H2, monoclonal
• faj reaktivitás: human
• kiszerelés: antibody small pack of 25 μg
• technika/technikák: immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotípus: IgG2bκ
• NCBI elérési szám: NP_000112
• UniProt elérési szám: P19235
• kiszállítva: ambient
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... EPOR(2057)

Leírás

Általános leírás

Erythropoietin receptor (UniProt: P19235; also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type 1 membrane protein that mediates erythropoietin-induced erythroblast proliferation and differentiation. It is synthesized with a signal peptide (aa 1-24) that is cleaved to produce the mature form. Upon EPO stimulation, EPO-R homodimerizes and gets phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins. The EPO-R is expressed in erythroid cells and erythroid progenitor cells. Three isoforms of EPO-R has been described that are produced by alternate splicing. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. Mutation in EPOR gene are linked to familial erythrocytosis that is characterized by elevated serum red blood cell mass, hemoglobin and hematocrit, and increased sensitivity of erythroid progenitors to erythropoietin.

Egyediség

Clone BCO-3H2 is a rat monoclonal antibody that detects erythopoietin receptor in human cells. It targets an epitope with in 17 amino acids from the C-terminal region.

Immunogen

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to 17 amino acids from the human erythropoietin receptor.

Alkalmazás

Anti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected EPO-R in Immunohistochemistry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Western Blotting Analysis: A representative lot detected EPO-R in Western Blotting applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Research Category
Signaling

Minőség

Evaluated by Western Blotting in UT-7 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected EPO-R in 10 µg of UT-7 cell lysates.

Cél megnevezése

~58 kDa observed; 55.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Fizikai forma

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in PBS without azide.

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable