MAB874

Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5

clone C1P5, Chemicon^®, from mouse

• Szinonimák: HPV
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: C1P5, monoclonal
• application: IHC; WB
• faj reaktivitás: human
• technika/technikák: immunohistochemistry: suitable; western blot: suitable
• citations: 12

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 300
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: C1P5, monoclonal
• faj reaktivitás: human
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: immunohistochemistry: suitable; western blot: suitable
• izotípus: IgG
• kiszállítva: wet ice

Leírás

Általános leírás

Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.

Egyediség

Recognizes Human papilloma virus type 18 early E6 protein. The antibody cross reacts with E6 protein from HPV type 16.

Immunogén

C1P5 was made against HPV type 18 E6 protein produced in bacteria.

Epitope: 16, 18 E6 protein

Alkalmazás

Detect Papillomavirus using this Anti-Papillomavirus Antibody, 16, 18 E6 protein, clone C1P5 validated for use in IH & WB.

Immunoblotting: Reactive with a 16.5-18 kDa band in reduced westerns of overexpressing cells lines corresponding to HPV E6 protein in cells derived from human cervical carcinoma. {Banks, et al 1987}

Immunohistochemistry: Shows reactivity on formalin fixed tissue. Dilute with buffer pH 7.5-8.0 to desired working volumes. For extensive dilution, protein containing or other stabilizing medium should be used.

Immunopreciptiation: Immunoprecipiates 16.5 kDa band from human cervical carcinoma extracts {Banks, et al, 1987}.

Final working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Cél megnevezése

16.5-18 kDa

Fizikai forma

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Tárolás és stabilitás

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analízis megjegyzés

Control
Human cervical carcinoma extract

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable