MAB345M
Anti-O4 Antibody, clone 81
clone 81 (mAB O4), Chemicon®, from mouse
Szinonimák:
Sulfatide
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
81 (mAB O4), monoclonal
application:
ICC
IHC
IHC
faj reaktivitás:
rat, mouse, human, chicken
technika/technikák:
immunocytochemistry: suitable
immunohistochemistry: suitable
immunohistochemistry: suitable
citations:
48
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
100
300
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
81 (mAB O4), monoclonal
faj reaktivitás
rat, mouse, human, chicken
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunocytochemistry: suitable
immunohistochemistry: suitable
izotípus
IgM
alkalmasság
not suitable for Western blot
not suitable for immunoprecipitation
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Egyediség
Recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm (see Additional Information library for list).
Alkalmazás
Anti-O4 Antibody, clone 81 is an antibody against O4 for use in IC, IH with more than 50 product citations.
Immunohistochemistry: 10-20 μg/mL on unfixed, shock frozen tissue.
Immunocytochemistry: 10-20 μg/mL on cells fixed with 4% paraformaldehyde.
Note: O4 is a sulfatide, which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry protocol
1. Prepare sections from unfixed, shock frozen tissue. The sections should be 4-5 μm thick. Place the sections on microscope slides.
2. Wash the slide three times for 5 min. each in PBS at room temperature.
3. Block the non-specific binding sites by incubating the sections in a humid chamber with 5% FCS at room temperature for 30 minutes.
4. Wash the slides as described in step 2.
5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.
6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.
7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein* solution and incubate in a humid chamber at 37°C for one hour.
8. Wash the slides as described in step 6.
9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.
*HRP or ABC can also be used.
Optimal results can be obtained by titrating the primary and secondary antibodies
Immunocytochemistry
1. Fix the preparations with 4% paraformaldehyde (in PBS) at room temperature for 10 minutes. O4 is a sulfatide which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.
2. Wash the slide three times for 5 min. each in PBS at room temperature.
3. Block the non-specific binding sites by incubating the sections in a human chamber with 5% FCS at room temperature for 30 minutes.
4. Wash the slides as described in step 2.
5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.
6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.
7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein solution and incubate in a humid chamber at 37°C for one hour.
8. Wash the slides as described in step 6.
9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.
Note: Do not allow the preparations to dry out during staining.
Immunocytochemistry: 10-20 μg/mL on cells fixed with 4% paraformaldehyde.
Note: O4 is a sulfatide, which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry protocol
1. Prepare sections from unfixed, shock frozen tissue. The sections should be 4-5 μm thick. Place the sections on microscope slides.
2. Wash the slide three times for 5 min. each in PBS at room temperature.
3. Block the non-specific binding sites by incubating the sections in a humid chamber with 5% FCS at room temperature for 30 minutes.
4. Wash the slides as described in step 2.
5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.
6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.
7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein* solution and incubate in a humid chamber at 37°C for one hour.
8. Wash the slides as described in step 6.
9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.
*HRP or ABC can also be used.
Optimal results can be obtained by titrating the primary and secondary antibodies
Immunocytochemistry
1. Fix the preparations with 4% paraformaldehyde (in PBS) at room temperature for 10 minutes. O4 is a sulfatide which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.
2. Wash the slide three times for 5 min. each in PBS at room temperature.
3. Block the non-specific binding sites by incubating the sections in a human chamber with 5% FCS at room temperature for 30 minutes.
4. Wash the slides as described in step 2.
5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.
6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.
7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein solution and incubate in a humid chamber at 37°C for one hour.
8. Wash the slides as described in step 6.
9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.
Note: Do not allow the preparations to dry out during staining.
Fizikai forma
Format: Purified
Purified immunoglobulin in 0.05M Potassium phosphate buffer, pH 8.0 with 0.3M NaCl and 0.05% sodium azide.
Analízis megjegyzés
Control
Rat cortical stem cells or day 3 cell cultures of brains from mouse embryos
Rat cortical stem cells or day 3 cell cultures of brains from mouse embryos
Egyéb megjegyzések
Concentration: Varies, see lot specific CoA
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Systemic injection of neural stem/progenitor cells in mice with chronic EAE.
Donega, M; Giusto, E; Cossetti, C; Schaeffer, J; Pluchino, S
Journal of Visualized Experiments null
Marc-André Mouthon et al.
Journal of neurochemistry, 99(3), 807-817 (2006-08-24)
Developing and adult forebrains contain neural stem cells (NSCs) but no marker is available to highly purify them. When analysed by flow cytometry, stem cells from various tissues are enriched in a 'side population' (SP) characterized by the exclusion of
Yifat Amir-Levy et al.
Multiple sclerosis international, 2014, 926134-926134 (2015-01-23)
Background. The neural stem cells (NSCs) migrate to the damaged sites in multiple sclerosis (MS) and in experimental autoimmune encephalomyelitis (EAE). However, the differentiation into neurons or oligodendrocytes is blocked. Epidermal growth factor (EGF) stimulates NSC proliferation and mobilization to
Differentiation of human breast-milk stem cells to neural stem cells and neurons.
Hosseini, SM; Talaei-Khozani, T; Sani, M; Owrangi, B
Neurology research international null
Zhuo Wang et al.
Development, growth & differentiation, 53(3), 357-365 (2011-04-12)
We attempted to test whether the differentiated NIH/3T3 fibroblasts could be differentiated into neuronal cells without any epigenetic modification. First, a neurosphere assay was carried out, and we successfully generated neurosphere-like cells by floating cultures of NIH/3T3 fibroblasts in neural
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással