MAB1621
Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11
clone 3H11, Chemicon®, from mouse
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
affinity isolated antibody
antitest terméktípus
primary antibodies
klón
3H11, monoclonal
tisztítva
affinity chromatography
faj reaktivitás
avian
faj reaktivitás (homológia által előrejelzett)
mammals
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotípus
IgG1
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... NEFM(4741)
Általános leírás
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
Egyediség
Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.
Immunogen
Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.
Alkalmazás
Anti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH.
Immunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections
Immunoblotting: 1:1000 - 1:2000 ECL detection.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:1000 - 1:2000 ECL detection.
Optimal working dilutions must be determined by end user.
Fizikai forma
Affinity purified antibody in PBS, 50% glycerol, and 5 mM sodium azide
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Involvement of TRPV2 activation in intestinal movement through nitric oxide production in mice.
Mihara, H; Boudaka, A; Shibasaki, K; Yamanaka, A; Sugiyama, T; Tominaga, M
The Journal of Neuroscience null
Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.
Thomas, AM; Shea, LD
Journal of Controlled Release : Official Journal of the Controlled Release Society null
Pierre-Yves Lozach et al.
The Journal of biological chemistry, 279(31), 32035-32045 (2004-05-29)
The molecular mechanisms involved in the hepatic tropism of hepatitis C virus (HCV) have not been identified. We have shown previously that liver-expressed C-type lectins L-SIGN and DC-SIGN bind the HCV E2 glycoprotein with high affinity (Lozach, P. Y., Lortat-Jacob
Robin M Perelli et al.
Development (Cambridge, England), 148(9) (2021-05-08)
Angiogenesis in the developing mammalian retina requires patterning cues from astrocytes. Developmental disorders of retinal vasculature, such as retinopathy of prematurity (ROP), involve arrest or mispatterning of angiogenesis. Whether these vascular pathologies involve astrocyte dysfunction remains untested. Here, we demonstrate
Chi-Yuan Chiu et al.
European journal of pain (London, England), 26(10), 2238-2256 (2022-09-14)
Kv4 channels are key components controlling neuronal excitability at membrane potentials below action potential thresholds. It remains elusive whether Kv4.1 participates in pain regulation. We raised a Kv4.1 antibody to map Kv4.1+ neurons in the superficial dorsal horn of the
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással