MAB1574

Anti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2

ascites fluid, clone 5TF1-1C2, Chemicon^®

• Szinonimák: Poly-Glu, PolyQ
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: 5TF1-1C2, monoclonal
• application: ELISA; ICC; IHC; IP; WB
• faj reaktivitás: human
• technika/technikák: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• citations: 178

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: ascites fluid
• antitest terméktípus: primary antibodies
• klón: 5TF1-1C2, monoclonal
• faj reaktivitás: human
• gyártó/kereskedő neve: Chemicon^®
• technika/technikák: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• izotípus: IgG1κ
• kiszállítva: dry ice
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

Huntington’s disease (HD) belongs to a family of polyglutamine diseases, which includes dentatorubralpallidoluysian atrophy (DRPLA), spinobulbar muscular atrophy (SBMA) and spinocerebellar ataxia (SCA) types 1–3, 6, 7 and 17. In these diseases, the non-pathogenic alleles contain fewer than approximately 35 consecutive glutamine repeats and encode a normal polyglutamine domain. In contrast, the pathogenic alleles usually contain 39 or more consecutive glutamine repeats. Higher repeat numbers lead to lower ages of onset. Patients with 40-60 glutamine repeats normally develop disease as adults, whereas patients with more than 60 repeats develop a juvenile onset disease. Each polyglutamine expansion disorder displays characteristic pathology, with neuronal loss evident in specific regions of the brain. HD results from expansions of a glutamine tract in a large cystolic protein known as huntingtin.

Egyediség

The epitope of MAB1574 was found to be a homopolymeric glutamine stretch. The original immunogen was the general transcription factor TATA Box-binding protein (TBP) which contains a 38-glns stretch (Lescure et al). Other polyglutamine-containing proteins are recognized by the MAB1574, notably those involved in several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington′s disease and spinocerebellar ataxia type 2, 3 and 7 (Trottier et al., 1995). Importantly, for proteins involved in these neurodegenerative disorders, MAB1574 showed remarkable property of detecting much better the pathological proteins that contain a polyglutamine expansion (37 glns) than the wild type proteins (Trottier et al., 1995). MAB1574 has been used to identify new neurodegenerative diseases caused by polyglutamine expansion and to help for cloning of the corresponding affected genes (Trottier 1995-1998; Imbert 1996; Stevanin 1996). MAB1574 is also able to detect intracellular inclusions, which is a hallmark of such diseases (Paulson, 1997).

Immunogén

N-terminal part of the human TATA Box Binding Protein (TBP).

Alkalmazás

Anti-Polyglutamine-Expansion Diseases Marker Antibody, clone 5TF1-1C2 is an antibody against Polyglutamine-Expansion Diseases Marker for use in ELISA, IC, IH(P), IP & WB.

ELISA: 1:1,000-1:20,000

Western Blot: 1:1,000-1:20,000

Immunohistochemistry on frozen and paraffin sections (human tissue): 1:1,000-1:20,000

Immunocytochemistry on transfected cells: 1:1,000-1:20,000 Immunoprecipitation: 1:1,000-1:20,000

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Fizikai forma

Ascites fluid containing no preservatives.

Unpurified

Tárolás és stabilitás

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analízis megjegyzés

Control
Huntigton′s Disease brain

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable