FCMAB101A4
Milli-Mark® Anti-BrdU Antibody, clone BU-1 Alexa Fluor™ 488 conjugated
clone Bu-1, Milli-Mark®, from mouse
Szinonimák:
BrdU
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
ALEXA FLUOR™ 488
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
Bu-1, monoclonal
faj reaktivitás
human
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Milli-Mark®
technika/technikák
flow cytometry: suitable
immunocytochemistry: suitable
izotípus
IgG2a
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Related Categories
Általános leírás
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody′s special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
Egyediség
Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Immunogen
Bromodeoxyuridine
Alkalmazás
Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Minőség
Evaluated by flow cytometry with Jurkat Cells.
Cél megnevezése
none given
Fizikai forma
Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Protein G Purified
Tárolás és stabilitás
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Analízis megjegyzés
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
Jogi információk
ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
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