208772

Calpain-1 Substrate II, Fluorogenic

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides.

• Szinonimák: Calpain-1 Substrate II, Fluorogenic, H-E(EDANS)PLF~AERK(DABCYL)-OH
• Tapasztalati képlet (Hill-képlet): C₇₂H₉₇N₁₇O₁₆S
• Molekulatömeg: 1488.71
• UNSPSC kód: 12352200
• NACRES: NA.77

Tulajdonságok

• Minőségi szint: 100
• Teszt: ≥97% (HPLC)
• Forma: lyophilized solid
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; desiccated (hygroscopic); protect from light
• szín: red
• oldhatóság: DMSO: 5 mg/mL
• fluoreszcencia: λ_ex ~505 nm; λ_em ~335 nm
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.

Biokémiai/fiziológiai hatások

Cell permeable: no

Primary Target
Calpain-1 Substrate

Product does not compete with ATP.

Reversible: no

Kiszerelés

Packaged under inert gas

Figyelmeztetés

Toxicity: Irritant (B)

Szekvencia

H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H

Fizikai forma

Supplied as a trifluoroacetate salt.

Feloldás

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Egyéb megjegyzések

Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable