Ugrás a tartalomra
Merck
Összes fotó(3)

Fontos dokumentumok

17-10131

Sigma-Aldrich

ChIPAb+ Phospho-CREB (Ser133) - ChIP Validated Antibody and Primer Set

from rabbit

Szinonimák:

active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.52
klón:
polyclonal
application:
ChIP
EMSA
IHC
IP
WB
faj reaktivitás:
rat, mouse, human, hamster
technika/technikák:
ChIP: suitable
electrophoretic mobility shift assay: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
10

biológiai forrás

rabbit

Minőségi szint

klón

polyclonal

faj reaktivitás

rat, mouse, human, hamster

gyártó/kereskedő neve

ChIPAb+
Upstate®

technika/technikák

ChIP: suitable
electrophoretic mobility shift assay: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotípus

IgG

NCBI elérési szám

UniProt elérési szám

kiszállítva

dry ice

Géninformáció

human ... CREB1(1385)

Related Categories

Általános leírás

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Phospho-CREB (Ser133) set includes the Phospho-CREB (Ser133) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 64 bp region of human cFos CRE. The Phospho-CREB (Ser133) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Phospho-CREB (Ser133)-associated chromatin.
CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

Egyediség

Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.

Immunogén

Epitope: phospho-Ser133
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.

Alkalmazás

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from 293T cells (5 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 4 µg of Normal Rabbit IgG, (Part No. P64B), or 4 µg of Anti-Phospho-CREB (Ser133) antibody (Part No. CS204400) and the Magna ChIP® A Kit (Cat. # 17-610).
Successful immunoprecipitation of Phospho-CREB (Ser133) antibody associated DNA fragments was verified by qPCR using ChIP Primers cFos CRE (Part No. CS203203) as a positive locus, and cFos downstream 4Kb as a negative locus (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Untreated (lane 1) and Forskolin-treated (Lane 2) NIH/3T3 lysates were resolved by SDS-PAGE, transferred to PVDF, and probed with anti-phospho-CREB (Ser133) (1:1,000 dilution).
Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates phosphorylated CREB (Figure 3).
Immunoprecipitation:
10 µL from a representative lot of this antibody was shown to immunoprecipitate phosphorylated CREB from 500 µg of cells treated with 50 mM forskolin.
Immunohistochemistry: A 1:1000 dilution of a representative lot detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
Electrophoretic Mobility Shift:
A representative lot of this antibody has been shown to gel shift by an independent laboratory.
Research Category
Signaling

Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
This ChIPAb+ Phospho-CREB (Ser133) -ChIP Validated Antibody & Primer Set (Antibody ChIP) conveniently includes the antibody & the specific control PCR primers.

Kiszerelés

25 assays per set. Recommended use: ~4 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).

Minőség

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 293T cells (5 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using either 4 μg of a Normal Rabbit IgG , or 4 μg of Anti-Phospho-CREB antibody and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of Phospho-CREB (Ser133) associated DNA fragments was verified by qPCR using ChIP Primers cFos CRE (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

Cél megnevezése

Detects CREB only when phosphorylated on Ser133 (~43 kDa). May recognize ATF-1 (~38 kDa) and CREM (~30 kDa) as the two proteins share significant homology to the phosphorylated immunizing peptide.

Fizikai forma

Anti-phospho-CREB (Ser133) (rabbit polyclonal), Part No. CS204400. One vial containing 100 µg of affinity purified rabbit serum in 0.1 M Tris-Glycine (pH 7.4) 0.15 M NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Store at -20°C.
Normal Rabbit IgG, Part No. PP64B. One vial containing 125 µg Rabbit IgG in 125 µL storage buffer containing 0.05% sodium azide.
Store at -20°C.
ChIP Primers cFos CRE, Part No. CS203203. One vial containing 75 μL of 5 μM of each primer specific for the cFos CRE. Store at -20°C.
FOR: GGC CCA CGA GAC CTC TGA GAC A
REV: GCC TTG GCG CGT GTC CTA ATC T
Format: Purified
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.

Tárolás és stabilitás

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analízis megjegyzés

Control
Includes negative control normal rabbit IgG and primers specific for human cFOS CRE.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

12 - Non Combustible Liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Valentina Salvi et al.
Oncotarget, 7(26), 39256-39269 (2016-10-27)
Lymph node expansion during inflammation is essential to establish immune responses and relies on the development of blood and lymph vessels. Previous work in mice has shown that this process depends on the presence of VEGF-A produced by B cells
Hyun Jun Jung et al.
Journal of the American Society of Nephrology : JASN, 29(5), 1490-1500 (2018-03-25)
Background Renal water excretion is controlled by vasopressin, in part through regulation of the transcription of the aquaporin-2 gene (Aqp2).Methods To identify enhancer regions likely to be involved in the regulation of Aqp2 and other principal cell-specific genes, we used
Yoshimichi Takeda et al.
Journal of the American Heart Association, 7(10) (2018-05-10)
DNA methylation is believed to be maintained in adult somatic cells. Recent findings, however, suggest that all methylation patterns are not stable. We demonstrate that stimulatory signals can change the DNA methylation status around transcription factor binding sites and a
J E Aguirre et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 29(9) (2017-04-26)
Abdominal pain is one of the major symptoms of inflammatory Bowel Disease (IBD). The inflammatory mediators released by colon inflammation are known to sensitize the afferent neurons, which is one of the contributors to abdominal pain. However, not all IBD
Zeeba D Kabir et al.
PloS one, 8(12), e84165-e84165 (2013-12-21)
Prenatal cocaine exposure has been shown to alter cognitive processes of exposed individuals, presumed to be a result of long-lasting molecular alterations in the brain. In adult prenatal cocaine exposed (PCOC) mice we have identified a deficit in recall of

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással