1.50451

SeQuant^™ ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 50 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

• UNSPSC kód: 41115709
• oszlop H × belső átmérő: 5 cm × 4.6 mm
• részecskeméret: 5 μm
• mátrix aktív csoport: ZIC-HILIC bonding phase
• pórusméret: 200 Å pore size
• Mátrix: fully porous particle

Tulajdonságok

• Terméknév: SeQuant^™ ZIC-HILIC^™ (5µm,200Å) PEEK 50 x 4.6 mm, suitable for HPLC
• material: PEEK-lined stainless steel column
• Minőségi szint: 100
• Ügynökség: suitable for USP L114
• termékcsalád: SeQuant^™
• Jellemzők: endcapped: no
• parameter: 500 bar pressure; 70 °C max. temp.
• technika/technikák: HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• oszlop H × belső átmérő: 5 cm × 4.6 mm
• Mátrix: fully porous particle
• mátrix aktív csoport: ZIC-HILIC bonding phase
• részecskeméret: 5 μm
• pórusméret: 200 Å pore size
• működési pH: 2-7.5
• elválasztástechnika: hydrophilic interaction (HILIC)
• tárolási hőmérséklet: no temp limit

Leírás

Általános leírás

SeQuant^™ ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.
The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar columns using a standard HPLC Instrument.
Thanks to their favorable retention and robust performance, SeQuant^™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^™ ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Analízis megjegyzés

Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40

Jogi információk

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany