07-888
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody
Upstate®, from rabbit
Szinonimák:
40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
WB
faj reaktivitás:
mouse, human
technika/technikák:
western blot: suitable
citations:
6
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
mouse, human
gyártó/kereskedő neve
Upstate®
technika/technikák
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
phosphorylation (pThr246)
Géninformáció
human ... AKT1S1(84335)
mouse ... Akt1S1(67605)
Általános leírás
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
Egyediség
Recognizes phosphorylated PRAS40 (Thr246)), Mr 40 kDa.
Immunogén
Peptide corresponding to amino acid region encompassing the human phospho-PRAS40 (Thr246).
Alkalmazás
Anti-phospho-PRAS40 (Thr246) (Proline-Rich AKT substrate) Antibody is an antibody against phospho-PRAS40 (Thr246) for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Minőség
Routinely evaluated by Western Blot on PDGF stimulated NIH3T3 cell lysates.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Western Blot Analysis:
A 1:1000 dilution of this lot detected phosphorylated PRAS40 (Thr246) in RIPA lysates of unstimulated and PDGF stimulated NIH3T3 cells.
Cél megnevezése
Approx. 40 kDa
Fizikai forma
Format: Purified
Purified rabbit polyclonal IgG in buffer containing PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide and 50% glycerol.
Analízis megjegyzés
Control
PDGF stimulated NIH3T3 cells.
PDGF stimulated NIH3T3 cells.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Paul M Titchenell et al.
Cell metabolism, 23(6), 1154-1166 (2016-05-31)
During insulin-resistant states such as type II diabetes mellitus (T2DM), insulin fails to suppress hepatic glucose production (HGP) yet promotes lipid synthesis. This metabolic state has been termed "selective insulin resistance" to indicate a defect in one arm of the
Anna E Garcia Whitlock et al.
Molecular metabolism, 51, 101246-101246 (2021-05-09)
Stress-induced hyperglycemia is associated with poor outcomes in nearly all critical illnesses. This acute elevation in glucose after injury or illness is associated with increased morbidity and mortality, including multiple organ failure. Stress-induced hyperglycemia is often attributed to insulin resistance
Regulation of Akt during torpor in the hibernating ground squirrel, Ictidomys tridecemlineatus.
McMullen DC, Hallenbeck JM
Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology null
Inositol polyphosphate multikinase is a physiologic PI3-kinase that activates Akt/PKB.
Maag, D; Maxwell, MJ; Hardesty, DA; Boucher, KL; Choudhari, N; Hanno, AG; Ma, JF; Snowman et al.
Proceedings of the National Academy of Sciences of the USA null
José L Areta et al.
The Journal of physiology, 591(9), 2319-2331 (2013-03-06)
Quantity and timing of protein ingestion are major factors regulating myofibrillar protein synthesis (MPS). However, the effect of specific ingestion patterns on MPS throughout a 12 h period is unknown. We determined how different distributions of protein feeding during 12
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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