07-626

Anti-phospho-AMPKα (Thr172) Antibody

Upstate^®, from rabbit

• Szinonimák: 5′-AMP-activated protein kinase, catalytic alpha-1 chain, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK alpha-1 chain, protein kinase, AMP-activated, alpha 1 catalytic subunit
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: WB
• faj reaktivitás: human
• technika/technikák: western blot: suitable
• citations: 15

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: human
• faj reaktivitás (homológia által előrejelzett): bovine, rat, chicken, mouse, horse
• gyártó/kereskedő neve: Upstate^®
• technika/technikák: western blot: suitable
• izotípus: IgG
• NCBI elérési szám: NP_996790
• UniProt elérési szám: Q13131
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: phosphorylation (pThr172)
• Géninformáció: human ... PRKAA1(5562) ; mouse ... Prkaa1(105787)

Leírás

Általános leírás

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

Egyediség

This antibody recognizes AMPKα.

Immunogén

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat.

Alkalmazás

Anti-phospho-AMPKα (Thr172) Antibody is an antibody against phospho-AMPKα (Thr172) for use in WB.

Research Category
Signaling

Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot (SNAP ID) Analysis:

Minőség

Evaluated by Western Blot in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected AMPKα in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

Cél megnevezése

62 kDa

Fizikai forma

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Tárolás és stabilitás

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analízis megjegyzés

Control
HEK293 cell lysate

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable