跳转至内容
Merck
  • Expression of activated STAT5 in neoplastic mast cells in systemic mastocytosis: subcellular distribution and role of the transforming oncoprotein KIT D816V.

Expression of activated STAT5 in neoplastic mast cells in systemic mastocytosis: subcellular distribution and role of the transforming oncoprotein KIT D816V.

The American journal of pathology (2009-11-07)
Christian Baumgartner, Sabine Cerny-Reiterer, Karoline Sonneck, Matthias Mayerhofer, Karoline V Gleixner, Richard Fritz, Marc Kerenyi, Cedric Boudot, Fabrice Gouilleux, Jan-Wilhelm Kornfeld, Christian Sillaber, Richard Moriggl, Peter Valent
摘要

Recent data suggest that the signal transducer and activator of transcription (STAT)5 contributes to differentiation and growth of mast cells. It has also been described that constitutively phosphorylated STAT5 (pSTAT5) plays a pro-oncogenic role in various myeloid neoplasms. We examined the expression of pSTAT5 in neoplastic mast cells in systemic mastocytosis and asked whether the disease-related oncoprotein KIT D816V is involved in STAT5 activation. As assessed by immunohistochemistry using the anti-pSTAT5 antibody AX1, neoplastic mast cells were found to display pSTAT5 in all SM patients examined (n = 40). Expression of pSTAT5 was also demonstrable in the KIT D816V-positive mast cell leukemia cell line HMC-1. Using various staining-protocols, pSTAT5 was found to be located in both the cytoplasmic and nuclear compartment of mast cells. To define the functional role of KIT D816V in STAT5-activation, Ba/F3 cells with doxycycline-inducible expression of KIT D816V were used. In these cells, induction of KIT D816V resulted in an increased expression of pSTAT5 without substantial increase in total STAT5. Moreover, the KIT D816V-targeting kinase-inhibitor PKC412 was found to counteract expression of pSTAT5 in HMC-1 cells as well as doxycycline-induced expression of pSTAT5 in Ba/F3 cells. Finally, a dominant negative STAT5-construct was found to inhibit growth of HMC-1 cells. Together, our data show that neoplastic mast cells express cytoplasmic and nuclear pSTAT5, that KIT D816V promotes STAT5-activation, and that STAT5-activation contributes to growth of neoplastic mast cells.

材料
货号
品牌
产品描述

Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, BioReagent, suitable for cell culture
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, cold ethanol fraction, pH 5.2, ≥96%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, pH 5.2, ≥96%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, suitable for RIA, pH 5.2, ≥96%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, crystallized, ≥98.0% (GE)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, low endotoxin, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, suitable for (for molecular biology), Non-acetylated
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, powder, BioXtra
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)
Sigma-Aldrich
抗磷酸酪氨酸抗体,克隆4G10®, clone 4G10®, Upstate®, from mouse
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, pH 5.2, ≥96%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, protease, essentially free, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, Essentially fatty acid free
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, pH <5.0, powder
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, essentially globulin free, BioReagent, suitable for cell culture
Sigma-Aldrich
牛血清白蛋白 来源于牛血清, lyophilized powder, ≥98% (agarose gel electrophoresis)