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生物源
bovine
化驗
≥98% (agarose gel electrophoresis)
形狀
lyophilized powder
分子量
~66 kDa
純化經由
heat shock fractionation
包裝
poly bottle of
來源
New Zealand origin
技術
flow cytometry: suitable
pH值
7
~7 (1% in 0.15 M NaCl)
溶解度
water: slightly soluble
UniProt登錄號
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
Bovine serum albumin (BSA) that belongs to the serum albumin family, is an α-helical, globular and non-glycosylated protein. It comprises three domains with two subdomains under each domain.
應用
Bovine Serum Albumin has been used:
- as a component of cryomedia for freezing testicular tissue
- to resuspend myometrial cells in flow cytometric analysis for cell viability quantification
- to block cardiac fibroblasts (CFs) for immunofluorescence staining
生化／生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones, and fatty acids. It displays high solubility and is exploited in the cosmetic and pharmaceutical industries. BSA is useful as a cell media component and favors embryonic stem cells (hESC)differentiation.
準備報告
来源于新西兰产地的牛血清
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Roman Franěk et al.
PloS one, 14(4), e0205481-e0205481 (2019-04-19)
Common carp (Cyprinus carpio) is one of the most cultured fish species over the world with many different breeds and plenty of published protocols for sperm cryopreservation. However, data regarding preservation of gonadal tissue and surrogate production is still missing.
Subhajit Ghosh et al.
The journal of physical chemistry. B, 119(25), 7804-7815 (2015-05-30)
The study of protein-surfactant interactions is important because of the widespread use of surfactants in industry, medicine, and pharmaceutical fields. Sodium N-lauroylsarcosinate (SL-Sar) is a widely used surfactant in cosmetics, shampoos. In this paper, we studied the interactions of bovine
S Chodankar et al.
Physical review. E, Statistical, nonlinear, and soft matter physics, 77(3 Pt 1), 031901-031901 (2008-06-04)
Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) have been used to study conformational changes in protein bovine serum albumin (BSA) due to perturbation in its native structure as induced by varying temperature and pressure, and in presence of
Geoffrey L Francis
Cytotechnology, 62(1), 1-16 (2010-04-08)
Albumin has a long historical involvement in design of media for the successful culture of mammalian cells, in both the research and commercial fields. The potential application of albumins, bovine or human serum albumin, for cell culture is a by-product
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
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