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Merck
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55261-U

Supelco

HybridSPE®-Phospholipid solid phase extraction (SPE) Cartridge

Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

别名:

HybridSPE (phospholipid and protein removal) SPE cartridge tube, 1 mL

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About This Item

分類程式碼代碼:
41115712
NACRES:
NB.21

product name

HybridSPE ®-磷脂SPE小柱, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

材料

PE frit (20 μm)
polypropylene hardware

產品線

HybridSPE®

成份

bed wt., 30 mg

包裝

pk of 100

技術

solid phase extraction (SPE): suitable

容積

1 mL

基質活性組

zirconia-based phase

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相关类别

一般說明

HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台，设计用于在 LC-MS 或 LC-MS/MS 分析之前，从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白，并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上，其作为化学过滤器，专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。

HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内，接着加入酸化乙腈（沉淀剂）。短暂混合/涡旋后，抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板，这些填充柱床的滤膜/筛板组件作为深层过滤器，有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。

應用


  • Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study assesses various extraction methods, including HybridSPE®-Phospholipid, for analyzing PFAS in tissues, highlighting efficiency and reproducibility (Mertens et al., 2023).

  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: This paper describes a rapid analytical method incorporating HybridSPE®-Phospholipid for sample preparation, demonstrating its application in environmental toxicology (Tanoue et al., 2020).


特點和優勢

  • 与离线蛋白沉淀联合使用。
  • 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
  • 通过完全除去磷脂和沉淀蛋白，减少离子抑制
  • 2-3 步通用流程
  • 方法开发最少，甚至不需要方法开发
  • 有 96 孔和 1 mL 萃取柱规格可供选择

法律資訊

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
18931401
18686101
18640301
18500901
18265401

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Junji Ohnishi et al.
Human genomics, 11(1), 21-21 (2017-09-04)
Growing evidence suggests that spiritual/religious involvement may have beneficial effects on both psychological and physical functions. However, the biological basis for this relationship remains unclear. This study explored the role of spiritual/religious involvement across a wide range of biological markers
Sara Anjos et al.
Journal of proteome research, 18(8), 3174-3183 (2019-07-11)
Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity.
Takahiko Mitsui et al.
International urology and nephrology, 52(2), 233-238 (2019-10-07)
The present study was conducted to identify metabolites using a metabolomics approach and investigate the relationship between these metabolites and urgency as a major symptom of overactive bladder (OAB). In 47 male participants without any apparent neurological disease, OAB was
Seiichiro Sakao et al.
Molecular medicine reports, 23(4) (2021-01-27)
It is generally considered that there is an increase in glycolysis in the hypertrophied right ventricle (RV) during pulmonary hypertension (PH), which leads to a decrease in glucose oxidation through the tricarboxylic acid (TCA) cycle. Although recent studies have demonstrated
Marie Bagge Jensen et al.
Food chemistry, 345, 128835-128835 (2020-12-16)
Growing evidence of vitamin K's importance in human health beyond blood coagulation and bone health necessitates its further research. A method involving extraction, lipase treatment, clean-up, and detection and quantification by LC-ESI-MS/MS of phylloquinone (PK), menaquinone-4 (MK-4), menaquinone-7 (MK-7) and
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商品

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

实验方案

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

相关内容

小分子HPLC

小分子为离子和化合物，分子量通常低于900道尔顿。这类化合物可使用柱化学性质各异的硅胶颗粒型或整体型固定相，通过HPLC、UHPLC和LC-MS等方法有效分离和分析。

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Chromatograms

application for HPLC

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