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技術
protein extraction: suitable
儲存溫度
−20°C
一般說明
植物总蛋白抽提试剂盒（Plant Total Protein Extraction Kit）是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提蛋白定性样本。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤（APTP）。本试剂盒包含两类试剂：一份植物特异性的蛋白酶抑制剂cocktail、一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂（C0356, 蛋白抽提试剂第4类试剂，Protein Extraction Reagent Type 4). 在植物组织样品顺序蛋白抽提过程中采用新鲜试剂，能洗脱植物组织中的几乎所有蛋白质。蛋白酶抑制剂cocktail是作用于丝氨酸、半胱氨酸、金属蛋白酶、天冬氨酸与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。本品含有胃酶抑素A，4-（2-氨基乙基）苯磺酰氟（AEBSF）中，E-64，亮肽素，1,10-菲咯啉，和苯丁抑制素等多种成分。经验证，cocktail抑制剂混合物能够在抽提过程中高效避免蛋白降解的发生。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用离液剂重悬。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用离液剂重悬。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
應用
植物总蛋白提取试剂盒用于分离种子粗蛋白。还用于提取总蛋白。
數量
足够用于从10-250mg的植物组织样品中制备20个单独的提取物
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
儲存類別代碼
11 - Combustible Solids
閃點（°F）
185.0 °F - closed cup
閃點（°C）
85 °C - closed cup
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