推荐产品
儲存溫度
2-8°C
一般說明
ProteoPrep® 膜提取试剂盒试剂采用强力去垢剂来提高2D凝胶的蛋白上样率和分辨率。3和4型Protein Extraction Reagent 针对蛋白质组研究进行了优化。3和4型提取试剂是在等电位聚焦和2D电泳之前进行蛋白提取的最理想产品。 本试剂盒包含进行二硫键还原及烷基化所需的试剂。ProteoPrep® 膜提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。
應用
ProteoPrep®膜提取试剂盒可从多种类型细胞制备高度富集的膜蛋白溶液。最终制得的蛋白溶液可用于2D凝胶电泳。
特點和優勢
- 创新的去垢剂制备方法 - 提高了溶解度,可加载更多的蛋白质,提高了2D凝胶中低丰度蛋白的可见性。
- 两种预混的增溶溶液 - 在提取前消除了干扰的非膜蛋白,提高了2D矩阵的整洁度。
- 预测定的还原剂和烷化剂 - 试剂易于使用,可提供更高的IEF分辨率。
法律資訊
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險分類
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
閃點(°F)
186.8 °F - closed cup
閃點(°C)
86 °C - closed cup
其他客户在看
Applied and environmental microbiology, 73(7), 2037-2047 (2007-02-06)
Escherichia coli O157:H7 causes hemorrhagic colitis and hemolytic-uremic syndrome in humans, and its major reservoir is healthy cattle. An F-like 92-kb plasmid, pO157, is found in most E. coli O157:H7 clinical isolates, and pO157 shares sequence similarities with plasmids present
Journal of proteomics, 75(8), 2488-2499 (2012-03-16)
Antarctic bacteria are adapted to the extremely low temperature. The transcriptional and translational machineries of these bacteria are adapted to the sub-zero degrees of temperature. Studies directed towards identifying the changes in the protein profiles during changes in the growth
Applied and environmental microbiology, 74(16), 5015-5022 (2008-06-17)
Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we
American journal of physiology. Renal physiology, 292(2), F599-F606 (2006-09-14)
Fetuin-A is a known inhibitor of vascular calcification in vitro. In arteries with calcification, there is increased immunostaining for fetuin-A. However, vascular smooth muscle cells (VSMC) do not synthesize fetuin-A, suggesting fetuin-A may be endocytosed to exert its inhibitory effects.
BMC microbiology, 10, 225-225 (2010-08-27)
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial
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