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儲存溫度
−20°C
一般說明
植物疏水蛋白抽提试剂盒（ Plant Hydrophobic Protein Extraction Kit ）是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中疏水蛋白的定性样品。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤（APTP）。本试剂盒包含三类试剂：一份植物特异性的蛋白酶抑制剂cocktail、一份用于抽提亲水蛋白质的专用配方试剂（植物蛋白抽提第1类试剂，Plant Protein Extraction Reagent Type 1），一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂(C0356, 蛋白抽提试剂第4类试剂，Protein Extraction Reagent Type 4）。蛋白酶抑制剂cocktail是作用于丝氨酸、半胱氨酸、金属蛋白酶、天冬氨酸与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。本品含有胃酶抑素A，E-64，亮肽素，4-（2-氨基乙基）苯磺酰氟（AEBSF）中，1,10-菲咯啉，和苯丁抑制素等多种成分。经验证，cocktail抑制剂混合物能够在抽提过程中高效避免蛋白降解的发生。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提亲水蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。最后用户可获得定性的疏水蛋白样品，用于下游蛋白组学分析。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提亲水蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。最后用户可获得定性的疏水蛋白样品，用于下游蛋白组学分析。
數量
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
儲存類別代碼
10 - Combustible liquids
閃點（°F）
185.0 °F
閃點（°C）
85 °C
Ramesh Sundar Amalraj et al.
Electrophoresis, 31(12), 1959-1974 (2010-06-22)
Sugarcane is an important commercial crop cultivated for its stalks and sugar is a prized commodity essential in human nutrition. Proteomics of sugarcane is in its infancy, especially when dealing with the stalk tissues, where there is no study to
Yassel Ramos et al.
Analytical biochemistry, 377(2), 134-140 (2008-04-04)
We investigate the selectivity achieved after differential solubilization of bacterial proteomes following two procedures, both based on successive extraction of proteins in solutions of increasing solubilizing power. Recently, these procedures have gained notable popularity and several commercial kits are now
M Cilia et al.
Journal of biomolecular techniques : JBT, 20(4), 201-215 (2009-09-02)
Protein extraction methods can vary widely in reproducibility and in representation of the total proteome, yet there are limited data comparing protein isolation methods. The methodical comparison of protein isolation methods is the first critical step for proteomic studies. To
Angus L Dawe et al.
Methods in molecular biology (Clifton, N.J.), 722, 225-236 (2011-05-19)
The interaction of the filamentous fungal plant pathogen Cryphonectria parasitica with its virulence-attenuating viruses provides a unique platform to explore the molecular biology and genetics of virus-host interactions. Following the development of transformation procedures for this fungus, subsequent advances include
Siew-Yong Koay et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(22), 2179-2183 (2011-06-22)
Orthosiphon aristatus is a traditionally used medicinal plant. In order to study the proteome of the plant, we have developed a simple plant protein extraction method by direct extraction of protein using a modified 2D-gel compatible tris-sucrose buffer followed by
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