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Merck

N5386

Sigma-Aldrich

微球菌核酸酶 来源于金黄色葡萄球菌

100-300 units/mg protein

别名:

MNase, 微球菌核酸内切酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Staphylococcus aureus

品質等級

形狀

powder

比活性

100-300 units/mg protein

成份

Protein, ≥40% E1%/280 (Balance primarily sodium acetate)

技術

DNA extraction: suitable
DNA purification: suitable

適合性

suitable for molecular biology

應用

cell analysis

儲存溫度

−20°C

基因資訊

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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應用

微球菌核酸酶可用于消化染色质以鉴定核小体的位置。
来源于金黄色葡萄球菌的微球菌核酸酶已被用于研究中监测与 DNA 的杂交反应。它也被用于研究葡萄球菌核酸酶测定的最佳条件。

生化/生理作用

可水解DNA的5′-磷酸二酯键。

其他說明

注意:1μmole单位 = 约85 A260 单位,其中A260单位相当于在pH 8.8,37℃条件下,在3.55 mL反应体积中,30分钟内ΔA260为1.0。光路= 1cm。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Luye Qin et al.
Nature communications, 12(1), 6589-6589 (2021-11-17)
ASH1L, a histone methyltransferase, is identified as a top-ranking risk factor for autism spectrum disorder (ASD), however, little is known about the biological mechanisms underlying the link of ASH1L haploinsufficiency to ASD. Here we show that ASH1L expression and H3K4me3
Mande Xue et al.
Molecular plant, 14(11), 1799-1813 (2021-07-10)
Global warming poses a major threat to plant growth and crop production. In some plants, including Arabidopsis thaliana, elevated temperatures induce a series of morphological and developmental adjustments termed thermomorphogenesis, which facilitates plant cooling under high-temperature conditions. Plant thermal response is

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