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Merck
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AMPD1

Sigma-Aldrich

脱氧核糖核酸酶I

Amplification Grade

别名:

脱氧核糖核酸酶 I（DNA酶I）

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About This Item

EC 号:
3.1.21.1 (BRENDA, IUBMB)
分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

濃度

1 unit/μL

技術

RT-PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

脱氧核糖核酸酶I（DNase I）是一种从牛胰腺中分离的内切核酸酶，可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性，适于在 RT-PCR（逆转录酶 - 聚合酶链反应）等敏感应用之前去除 RNA 制剂中的DNA。

DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。

当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。

應用

扩增级脱氧核糖核酸酶I（DNase I）已用于：
  • 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
  • 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
  • 从牛血样中提取的总 RNA 中去除污染 DNA

特點和優勢

  • 适用于去除 RNA 中的 DNA
  • 超低RNase活性
  • 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活

適合性

适用于去除 RNA 制剂中的 DNA。

單位定義

在 37 °C 下，一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。

法律資訊

购买本产品附带有限的许可，仅可用于研究用途聚合酶链式反应 (PCR) 过程，且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖，该费用通过向 Applied Biosystems 支付或购买的方式（即授权的热循环仪）支付。

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儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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