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Merck

M5755

Sigma-Aldrich

MOPS-EDTA-乙酸钠 (MESA)

powder for RNA electrophoresis buffers

别名:

MESA缓冲液

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

品質等級

產品線

BioReagent

形狀

powder

技術

electrophoresis: suitable

pH值

6.8-7.2(reconstituted per directions)

溶解度

H2O: soluble, faintly yellow

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

room temp

一般說明

MOPS-EDTA-乙酸钠(MESA)是Northern印迹程序之前RNA电泳最常用的运行缓冲液。 对于最大值分离度,建议施加5 V/cm的电压。

應用

适合在制备甲醛-琼脂糖凝胶和RNA电泳相关的运行缓冲液时使用。

成分

1x MESA 缓冲液包含:
  • 40 mM MOPS
  • 10 mM乙酸钠
  • 1 mM EDTA (pH 8.3)

重構

将瓶中的全部内容物溶解在最终体积为1L的分子生物学级水中。 这产生了10X MESA贮备液,可以根据需要进一步稀释。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)

实验方案

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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