跳转至内容
Merck
所有图片(1)

文件

B6429

Sigma-Aldrich

酪蛋白封闭缓冲液 10x

for Northern and Southern blotting, solution

别名:

10x Blocking Buffer

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
12161700
NACRES:
NA.54

等級

for molecular biology

形狀

solution

技術

Southern blotting: suitable

儲存溫度

room temp

相关类别

一般說明

酪蛋白是存在于乳汁中的磷蛋白。它分为四种亚型，包括αS1-、αS2-、β-和 κ-酪蛋白。
Sigma 的 10x 封闭缓冲液是一种基于酪蛋白的封闭剂，常规用于减少检测试剂与 Northern 和 Southern 印迹膜的非特异性结合。

應用

酪蛋白封闭缓冲液10X已用于：

  • 蛋白印迹
  • 酶联免疫吸附分析(ELISA)
  • 免疫组化

特點和優勢

  • 封闭性能优于奶粉或 BSA
  • 易于使用的浓缩液
  • 与中性和正电荷膜兼容
  • 与化学发光和比色检测试剂盒兼容

準備報告

使用前用分子生物学级水稀释 10x 封闭缓冲液。

象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

M B Engelund et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
查看所有相关文献

实验方案

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门