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一般說明
核苷酸是有机分子，可作为核酸（如DNA和RNA）的单体或亚基。核苷酸是核酸的结构单元，由一个含氮碱基（嘌呤或嘧啶）、一个五碳糖（核糖或脱氧核糖）和至少一个磷酸基团组成。一个核苷与一个磷酸基团组合形成一个核苷酸。脱氧核苷酸混合物是一种便捷式预混dNTP溶液，以高质量分子生物学级水为溶剂，含有等摩尔（10mM）的UltraPure超纯dATP、dCTP、dGTP和TTP钠盐。1 µL足够进行一次标准50 µL PCR反应。
應用
dNTP混合物已用于：
- PCR扩增昆虫、 真菌、病毒、人类来源分离的基因组DNA
- 总RNA逆转录为cDNA
- 作为聚合酶链式反应(PCR)DNA扩增混合物组分
- 常规和长片段PCR
- 手动和自动DNA测序
- cDNA合成和标记反应
特點和優勢
- 每种dNTP的纯度：最低99%
- 方便配制；每50 μL PCR使用1 μL
- 等摩尔量的各种dNTP可减少移液步骤
- 最大限度地降低PCR污染风险
- UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.
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