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Key Documents

93017

Sigma-Aldrich

SSC缓冲液20倍浓缩液

for Northern and Southern blotting, solution

别名:

20x SSC

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

品質等級

產品線

BioReagent

形狀

solution

技術

Southern blotting: suitable

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH值

7.0±0.2

密度

1.16 g/mL at 20 °C

儲存溫度

room temp

一般說明

SSC缓冲液20倍浓缩液是Southern和Northern印迹法的标准试剂。该浓缩配方可直接使用,或稀释后制成浓度低至0.5倍的SSC工作溶液。

應用

适用于:
  • 制备用于杂交的核酸
  • 制备用于Northern和Southern印迹的杂交缓冲液
  • 制备用于Northern和Southern印迹的洗涤缓冲液

特點和優勢

  • 方便的即用型液体
  • 在杂交中具有多种用途
  • 可轻松稀释至所需浓度

成分

SSC缓冲液20倍浓缩液的溶剂为含有0.3 M柠檬酸钠的3M NaCl。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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实验方案

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

相关内容

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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