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Merck

11096176001

Roche

封闭试剂

For nucleic acid hybridization and detection

别名:

封闭剂

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About This Item

分類程式碼代碼:
41116100

品質等級

形狀

powder

包裝

pkg of 50 g

製造商/商標名

Roche

儲存溫度

20-25°C

應用

该封闭试剂用于在非放射性滤膜杂交以及核酸杂交物的检测中降低背景。它也在免疫组化中用作封闭剂。

準備報告

工作浓度:抗体稀释1:5,000
工作溶液:如需制备10x封闭储备溶液,封闭试剂可溶于马来酸缓冲液中至10%(w/v)终浓度,并伴随晃动以及在加热模块或微波炉中加热。
储备条件(工作溶液):封闭试剂的10x储备溶液经高温灭菌后2至8℃储存。或者,在分装后-15至-25℃储存。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Revised lineage of larval photoreceptor cells in Ciona reveals archetypal collaboration between neural tube and neural crest in sensory organ formation.
Oonuma K, et al.
Developmental Biology, 420(1), 178-185 (2016)
Nodal promotes functional luteolysis via down-regulation of progesterone and prostaglandins E2 and promotion of PGF2α synthetic pathways in mare corpus luteum.
Galv?o A, et al.
Endocrinology, 157(2), 858-871 (2015)
Nuria de Pedro et al.
Biological procedures online, 22, 2-2 (2020-01-21)
A large number of studies have suggested a correlation between the status of telomeres and disease risk. High-throughput quantitative fluorescence in situ hybridization (HT Q-FISH) is a highly accurate telomere measurement technique that can be applied to the study of
Richard C Krolewski et al.
Scientific reports, 10(1), 1781-1781 (2020-02-06)
The family of olfactory receptors (ORs) subserves the sense of smell and includes both functional alleles and pseudogenes, the latter identified by mutations resulting in frame shift or premature truncation. During neuronal differentiation, nonfunctional ORs are expressed initially but then
Kyle A Wegner et al.
The Journal of pathology, 250(2), 231-242 (2019-11-02)
We genetically engineered expression of an activated form of P110 alpha, the catalytic subunit of PI3K, in mouse prostate epithelium to create a mouse model of direct PI3K activation (Pbsn-cre4Prb;PI3KGOF/+ ). We hypothesized that direct activation would cause rapid neoplasia

实验方案

The easiest way to use the Blocking Reagent is with the Roche DIG Wash and Block Buffer Set, a ready-to-use set of stock solutions.

使用封闭剂最简单的方法是使用Roche DIG Wash and Block Buffer 套装,这是一套即用型储液。

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