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Merck
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文件

S0902

Sigma-Aldrich

SSC缓冲剂

for Northern and Southern blotting, powder blend

别名:

柠檬酸钠盐缓冲液

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

品質等級

200

形狀

powder blend

pH值

6.8-7.2 (20-25 °C, 263 mg/L in water)

溶解度

water: 263 mg/mL, clear, colorless

異物活動

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

儲存溫度

room temp

相关类别

一般說明

SSC缓冲液20x粉末是Southern和Northern blotting的一种标准试剂。该浓缩后的粉末应在使用前用分子生物学级水重构至指定的最终体积（终溶液为20x）。该浓缩液可直接使用，或稀释后制成浓度低至0.5x的SSC工作溶液。

應用

柠檬酸钠盐（SSC）缓冲液在微阵列技术中已被用作洗涤液。它也被用作荧光原位杂交（FISH）缓冲液中的一种组分。
适用于：
  • 制备用于杂交的核酸
  • 制备用于Northern和Southern印迹的杂交缓冲液
  • 制备用于Northern和Southern印迹的洗涤液

特點和優勢

  • 在杂交中具有多种用途
  • 可简单的被重构并稀释成任何所需的浓度

成分

20x的SSC缓冲液终浓缩液在3M NaCl中含有0.3 M柠檬酸钠。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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At the time of fertilization, the paternal genome lacks the typical configuration and marks characteristic of pericentric heterochromatin. It is thus essential to understand the dynamics of this region during early development, its importance during that time period and how

实验方案

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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