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品質等級
形狀
liquid (2x concentrate)
異物活動
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
儲存溫度
−20°C
一般說明
Sigma的杂交液是一种2倍即用型杂交缓冲液,适用于Northern和Southern印迹方案。
應用
杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。
成分
Sigma的杂交液含为10倍SSC和2倍Denhardt溶液中的200 μg/mL DNA:
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg聚蔗糖
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg聚蔗糖
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl
相關產品
产品编号
说明
价格
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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