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Merck

50980

Sigma-Aldrich

异硫氰酸胍

BioUltra, for molecular biology, ≥99.0% (AT)

别名:

硫氰酸亚脲, 硫氰酸胍

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About This Item

线性分子式:
NH2C(=NH)NH2 · HSCN
CAS号:
分子量:
118.16
Beilstein:
3563461
EC號碼:
MDL號碼:
分類程式碼代碼:
12352107
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

產品線

BioUltra

化驗

≥99.0% (AT)

形狀

solid

雜質

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

燃燒殘留物

≤0.5% (as SO4)

損耗

≤0.1% loss on drying, 20 °C (HV)

pH值

4.5-7.0 (25 °C, 6 M in H2O)

mp

117-120 °C
120-122 °C (lit.)

溶解度

H2O: 6 M at 20 °C, clear, colorless

負離子痕跡

chloride (Cl-): ≤5000 mg/kg
sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

吸收

cut-off at 280 nm in H2O at 6 M

SMILES 字串

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI 密鑰

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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應用

离液剂和强效变性剂;溶解细胞。

其他說明

RNA 的分离 ;从富含核糖核酸酶和蛋白聚糖的组织中提取全长 RNA 模板进行逆转录

象形圖

CorrosionExclamation mark

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

安全危害

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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J C Groppe et al.
Analytical biochemistry, 210(2), 337-343 (1993-05-01)
RNA isolated by conventional guanidinium isothiocyanate methods from tissues of a mollusc (red abalone: Haliotis rufescens) is largely degraded and discolored by contaminants. These contaminants are associated with inhibition of reverse transcriptase, prevent accurate spectrophotometric determination of RNA concentration, and
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is
Turpen, T.H. and Griffith, O.M.
Biotechniques, 4, 11-11 (1986)
Kai Y Mak et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(9), 1434-1443 (2015-05-23)
Angiotensin converting enzyme 2 (ACE2) which breaks down profibrotic peptide angiotensin II to antifibrotic peptide angiotensin-(1-7) is a potential therapeutic target in liver fibrosis. We therefore investigated the long-term therapeutic effect of recombinant ACE2 using a liver-specific adeno-associated viral genome
Olesya V Stepanenko et al.
PloS one, 7(11), e48809-e48809 (2012-11-13)
Having a high folding efficiency and a low tendency to aggregate, the superfolder GFP (sfGFP) offers a unique opportunity to study the folding of proteins that have a β-barrel topology. Here, we studied the unfolding-refolding of sfGFP that was induced

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