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Merck
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文件

SRE0066

Sigma-Aldrich

盐酸胍溶液，6M

别名:

盐酸氨基甲脒, 盐酸胍, 胍盐酸盐

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About This Item

分類程式碼代碼:
12352209
NACRES:
NA.25

形狀

liquid

品質等級

500

濃度

6 M

應用

diagnostic assay manufacturing

儲存溫度

room temp

相关类别

應用

盐酸胍常用作变性剂，因为它能使氨基酸残基间氢键断裂。通过断开这些键，蛋白质的 3D 构象被展开，蛋白质的水溶性大大增加。一旦变性，可通过各种程序轻松还原、修饰或分析蛋白质。样品的进一步处理可以使蛋白质通过其他常见的蛋白质分析方法进行分析，包括质谱、电泳和酶消化。
盐酸胍通常也用于 RNA 的分离、球状蛋白质的变性和蛋白质复性研究。也可用于促进胰蛋白酶肽的生成，用于复杂蛋白样品的分析。

外觀

本品为即用型 6 M 盐酸胍溶液。6 M 盐酸胍溶液可用作储备液，制备以下溶液： 适当降低浓度。

注：如果 6 M 缓冲液中存在沉淀，则将瓶加热至 37°C，混合直至完全溶解后使用。

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H302,H315,H319

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Jun-Yi Zhu et al.
Autophagy, 17(10), 3160-3174 (2021-01-07)
We investigated in larval and adult Drosophila models whether loss of the mitochondrial chaperone Hsc70-5 is sufficient to cause pathological alterations commonly observed in Parkinson disease. At affected larval neuromuscular junctions, no effects on terminal size, bouton size or number
Georgios I Laliotis et al.
Nature communications, 12(1), 4624-4624 (2021-08-01)
AKT-phosphorylated IWS1 regulates alternative RNA splicing via a pathway that is active in lung cancer. RNA-seq studies in lung adenocarcinoma cells lacking phosphorylated IWS1, identified a exon 2-deficient U2AF2 splice variant. Here, we show that exon 2 inclusion in the
Kaamini M Dhanabalan et al.
Biomaterials science, 8(15), 4308-4321 (2020-07-01)
Osteoarthritis (OA) is a joint disease characterized by progressive damage of articular cartilage and the adjoining subchondral bone. Chondrocytes, the primary cells of the cartilage, have limited regenerative capacity and when they undergo stress due to trauma or with aging
Yoshinori Aso et al.
eLife, 8 (2019-11-15)
Animals employ diverse learning rules and synaptic plasticity dynamics to record temporal and statistical information about the world. However, the molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of the insect mushroom body function as parallel
Jingwen Yang et al.
Science signaling, 14(665) (2021-01-14)
Cranial neural crest cells (CNCCs) are a population of multipotent stem cells that give rise to craniofacial bone and cartilage during development. Bone morphogenetic protein (BMP) signaling and autophagy have been individually implicated in stem cell homeostasis. Mutations that cause

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