SOLu-Trypsin Rapid Digestion Kit (MSKT0002) consists of 400 ug of solution-stable trypsin (EMS0004) suitable for protein sequencing, and 30 mL of Rapid Digestion Buffer (EMS0009), allowing for trypsin digestion in one hour at 60°C. Trypsin should be used at a 1:10 enzyme:substrate ratio in Rapid Digestion Buffer, which can be substituted into existing user workflows.
Características y beneficios
Provides a fast and efficient method for protein digestion.
Removal of denaturants by offline desalting is not necessary, which further shortens the time for sample preparation.
Compatible with a variety of sample types, reduction and alkylation protocols, and downstream workflows.
Reliable and reproducible results in mass spectrometry proteomics analyses are highly dependent on robust sample preparation.
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