PP0100

Trypsin Profile IGD Kit

Name: Trypsin Profile IGD Kit
Brand: SIGMA

Sinónimos:

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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NACRES:

NA.56

UNSPSC Code:

12352200

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usage

kit sufficient for ≤100 applications

storage temp.

2-8°C

Quality Level

200

Categorías relacionadas

Protein Biology

Protein Mass Spectrometry

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Este artículo	T7575	TT0010	EMS0005
Sigma-Aldrich PP0100 Trypsin Profile IGD Kit	Sigma-Aldrich T7575 Trypsin Singles, Proteomics Grade	Sigma-Aldrich TT0010 Trypsin Spin Columns	Sigma-Aldrich EMS0005 SOLu-Trypsin Dimethylated
usage kit sufficient for ≤100 applications	usage -	usage -	usage -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

General description

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.[1][2]

Features and Benefits

Faster destaining than with alternative in-gel digest kits
Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
Compatible with Coomassie, SYPRO^™ Orange, SYPRO Ruby, and ProteoSilver^™ Stained gels
Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

Solo componentes del kit

Referencia del producto

Descripción

Destaining Solution Reconstituted to 75

pictograms

GHS02,GHS08,GHS07

signalword

Danger

hcodes

H225,H302 + H312 + H332,H315,H319,H334,H335

pcodes

P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

3 - Flammable liquids

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup

wgk

WGK 2

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Technical Bulletin - PP0100

Integrated Solutions for MALDI-MS

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