T7575

Trypsin Protease

Single-use aliquots ensuring consistent proteolytic activity, suitable for mass spectrometry

• Sinónimos: Proteomics grade, Trypsin, TPCK treated
• UNSPSC Code: 12352204
• NACRES: NA.56
• Specific activity: ≥10,000 BAEE units/mg protein

Propiedades

• Nombre del producto: Trypsin Singles, Proteomics Grade,
• grade: Proteomics Grade
• Quality Segment: 200
• form: ready-to-use solution
• specific activity: ≥10,000 BAEE units/mg protein
• mol wt: average mol wt 23.29 kDa
• optimum pH: ~8.0
• storage temp.: 2-8°C

Descripción

General description

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment.[1] Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Application

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry).[2] It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.[3]

Features and Benefits

• Convenient, single-use 1 μg size
• Eliminates repetitive pipetting
• Reductively methylated to minimize autolytic activity
• TPCK treated to quench chymotryptic activity
• Extensively purified

Los componentes del kit también están disponibles por separado

• Trypsin Solubilization Reagent

  SDS

Información de seguridad

• pictograms: GHS02,GHS08,GHS07
• signalword: Danger
• hcodes: H225,H302 + H312 + H332,H315,H319,H334,H335
• pcodes: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Clase de almacenamiento: 3 - Flammable liquids
• wgk: WGK 2