This product has not been tested for stability once dissolved in water. It is recommended to reconstitute just before use.
L5900
Linoleic Acid-Water Soluble
powder, BioReagent, suitable for cell culture
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10 MG
98,10 €
About This Item
UNSPSC-Code:
12352211
NACRES:
NA.75
Empfohlene Produkte
Biologische Quelle
plant (Safflower oil)
Produktlinie
BioReagent
Form
powder
Methode(n)
cell culture | mammalian: suitable
Löslichkeit
H2O: 50 mg/mL
Lagertemp.
−20°C
Anwendung
Linoleic acid-water soluble has been used as a component of fatting media for the in vitro steatosis induction of primary human hepatocytes (PHH).[1]
Biochem./physiol. Wirkung
Linoleic acid (LA) is an arachidonic acid precursor,[2] which is present in vegetable oils.[3] It enhances wound healing in diabetic rats.[2] LA triggers tumor and apoptosis in colorectal cancer cells.[3] The levels of LA in adipose tissue and platelets are associated with coronary artery disease.[4] Linoleic acid binds to molecules, such as bovine serum albumin (BSA)[5] or cyclodextrin for use in cell culture.[6]
Sonstige Hinweise
Contains approx. 30 mg of linoleic acid per gram; balance methyl-β-cyclodextrin.
Package size based on linoleic acid content.
Package size based on linoleic acid content.
Ähnliches Produkt
Signalwort
Warning
H-Sätze
P-Sätze
Gefahreneinstufungen
Eye Irrit. 2
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Kunden haben sich ebenfalls angesehen
Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production
Cell Culture Engineering IV, 51-56 (1994)
Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells
Pei F, et al.
Stem Cell Research, 19, 94-103 (2017)
B R Abdel-Halim
Theriogenology, 114, 143-148 (2018-04-07)
The current study was conducted to investigate the effect of linoleic acid-treated oocytes during in vitro maturation (IVM) on cumulus cell expansion, oocyte nuclear maturation, blastocyst development and DNA damage of cumulus cells and how its effects were alleviated by supplementation
An overview of the effect of linoleic and conjugated-linoleic acids on the growth of several human tumor cell lines
Maggiora M, et al.
International Journal of Cancer. Journal International Du Cancer, 112(6), 909-919 (2004)
Omega-6 vegetable oils as a driver of coronary heart disease: the oxidized linoleic acid hypothesis
DiNicolantonio JJ and O?Keefe JH
Open heart, 5(2), e000898-e000898 (2018)
Artikel
Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
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If we dissolve the powder in distilled water, as described in one of the questions, can we aliquot this solution and freeze it at -20 or -70. If so, for how long? Or should we make a fresh solution every time?
1 Antwort-
Hilfreich?
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Hi there, could you confirm the validity of our stock preparation for L5900-10MG? We dissolve the full 10 mg tube in 1.43 mL distilled water, yielding a 25 millimolar stock solution.
1 Antwort-
Linoleic acid has a molecular weight of 280.45 g/mol. Dissolving the full 10 mg tube in 1.43 mL of distilled water yields a 25 millimolar stock solution.
Hilfreich?
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Dear, can you please clarify the composition of this product for me? In my understanding this product contains minimum 2.5% linoleic acid encapsulated in methyl-β-cyclodextrin.
1 Antwort-
The product contains approximately 30 mg of linoleic acid per gram; balance methyl-?-cyclodextrin.
Package size is based on linoleic acid content.More information can be found in the Data Sheet below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdfHilfreich?
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How to prepare FAME from it?
1 Antwort-
This product is designed for use in mammalian cell culture applications as a supplement. If you are seeking a protocol for derivatization of Fatty Acids to FAMEs, please see the following: https://www.sigmaaldrich.com/technical-documents/protocol/food-and-beverage-testing-and-manufacturing/chemical-analysis-for-food-and-beverage/derivatization-of-fatty-acids-to-fames
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