An essential product for immunoprecipitations of VSV-tagged recombinant proteins. Given the packaging of the product, we aliquoted it into microfuge tubes on arrival and stored it at 4 C.
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Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
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clone P5D4, purified from hybridoma cell culture, PBS suspension
Synonym(e):
Monoclonal Anti-VSV Glycoprotein
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1 ML
779,00 €
779,00 €
Voraussichtliches Versanddatum17. April 2025
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1 ML
779,00 €
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.56
779,00 €
Voraussichtliches Versanddatum17. April 2025
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Konjugat
agarose conjugate
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
P5D4, monoclonal
Form
PBS suspension
chemische Klasse(n) des Analyten
proteins (VSV-G)
Methode(n)
immunoprecipitation (IP): suitable
protein purification: suitable
Isotyp
IgG1
Kapazität
≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
Versandbedingung
wet ice
Lagertemp.
2-8°C
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Allgemeine Beschreibung
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
Spezifität
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
Immunogen
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
Anwendung
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
Physikalische Form
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Angaben zur Herstellung
Prepared using cyanogen bromide-activated agarose.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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