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Merck

I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

Synonym(e):

Octylphenoxy-Poly(ethylenoxy)ethanol, verzweigt, Octylphenoxy poly(ethyleneoxy)ethanol, branched

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50 ML
128,00 €
100 ML
239,00 €

128,00 €


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50 ML
128,00 €
100 ML
239,00 €

About This Item

Lineare Formel:
(C2H4O)nC14H22O
CAS-Nummer:
UNSPSC-Code:
12352104
NACRES:
NA.31

128,00 €


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Qualität

for molecular biology

Qualitätsniveau

Beschreibung

non-ionic

Form

liquid

Methode(n)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

Fremdaktivität

DNase and RNase, none detected

SMILES String

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChIKey

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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Allgemeine Beschreibung

IGEPAL® CA-630 ist ein nichtionisches, nicht denaturierendes Detergens, das für die Solubilisierung, Isolation und Aufreinigung von Membranproteinkomplexen geeignet ist. Es ist chemisch nicht von Nonidet P-40 unterscheidbar.[1]

Anwendung

IGEPAL® CA-630 wurde verwendet
  • als neutrales Detergens im Homogenisierungspuffer beim Western Blotting von Mausmuskel-Homogenaten[2]
  • in der X-Gal-Färbung zur Untersuchung der LacZ-Expression in E8.5-E10.5-Embryos[3]
  • in Zell-Lyse-Puffer für die Zubereitung von einsatzbereiten Zelllysaten für RT-qPCR[4]

Sonstige Hinweise

Nichtionisches, nicht-denaturierendens Detergens

Qualität

Chemisch nicht unterscheidbar von Nonidet P-40, das nicht mehr im Handel erhältlich ist.

Vorsicht

Kann sich während der Lagerung eintrüben oder absetzen. Durch Erhitzen auf 40 °C erhält man eine klare Flüssigkeit.

Rechtliche Hinweise

IGEPAL is a registered trademark of Solvay

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

483.8 °F - closed cup

Flammpunkt (°C)

251 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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Kunden haben sich ebenfalls angesehen

Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Simon Lalonde et al.
Genome biology, 20(1), 133-133 (2019-07-10)
Genome-wide association studies (GWAS) have identified hundreds of loci associated with coronary artery disease (CAD) and blood pressure (BP) or hypertension. Many of these loci are not linked to traditional risk factors, nor do they include obvious candidate genes, complicating

Protokolle

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Auf dieser Seite finden Sie Informationen über verschiedene Pulldown-Assays für die weitere Isolation von Multiproteinkomplexen zur Identifizierung ihrer Komponenten mit Produkten von Cytiva.

Verwandter Inhalt

Untersuchen Sie Protein-Protein-Interaktionen im Reagenzglas mithilfe von Pulldown-Assays, bei denen Methoden wie Affinität, GST-Pulldown, TAP und Co-Immunpräzipitation zum Einsatz kommen.

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Fragen

1-10 von 17 Fragen  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 Antwort
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Hilfreich?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 Antwort
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Hilfreich?

  3. In which solvent(s) is Atazanavir Sulfate soluble?

    1 Antwort
    1. Atazanavir Sulfate is soluble in DMSO: 104mg/mL and EtOH: 20mg/mL.

      Hilfreich?

  4. What is the storage temperature for this item?

    1 Antwort
    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

      Hilfreich?

  5. Hi, I am enquiring about the storage temperature of product IGEPAL(R) CA-630 (I8896-100ml). The storage class is listed in the SDS but no temperature upper and lower limits. Would I be able to request this information. Thank you

    1 Antwort
    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, and the Safety Data Sheet (SDS).

      Hilfreich?

  6. What is the molecular weight of Product No. I8896, IGEPAL® CA-630?

    1 Antwort
    1. The approximate molecular weight of this product is 603 Daltons. The average EO (ethylene oxide) units per molecule are 9.

      Hilfreich?

  7. What is the wt/vol % of this IGEPAL reagent? I want to use it for ATAC-seq and in the protocols people always mention the concentration of the stock is 10% wt/vol but I can't find this information on the specifications of this product. Thanks

    1 Antwort
    1. This product is provided as a neat liquid with a concentration of 100% which can be further diluted. The density of IGEPAL CA-630 is 1.06 g/cm3 at 25 °C. For a 10% wt./vol. solution 9.43 mL IGEPAL is needed per 100 mL of solution.

      The product is generally stable based on the chemical composition and a 10% (w/v) aqueous solution may be stable refrigerated for at least one month. However, it is important to minimize the exposure of the solution to oxygen (keep closed soon after use). The product does have a tendency to oxidize in air forming peroxides through a free radical process. In addition, some bacterial growth may occur which is evidenced by an odor or formation of a precipitate. Preservatives added to the solution may prevent bacterial growth. Please view the data sheet below: https://www.braskem.com.br/Portal/Principal/Arquivos/html/boletm_tecnico/PP%20Chemical%20Resistance.pdf

      Hilfreich?

  8. What is the viscosity of Product No. I8896, IGEPAL® CA-630?

    1 Antwort
    1. The viscosity is determined for each lot and the information is available on the lot- specific Certificate of Analysis.

      Hilfreich?

  9. Are the 2 products, Igepal CA-630 (Product No. I8896) and Nonidet 40 the same? How do you use this product?

    1 Antwort
    1. Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.

      Hilfreich?

  10. What is the formula weight of Product No. I8896, IGEPAL® CA-630?

    1 Antwort
    1. The formula weight of this product is 603.

      Hilfreich?

1-10 von 17 Fragen  

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