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50879-U

Supelco

Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

L × I.D. 10 cm × 3 mm UHPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Ascentis® Express F5, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 3 mm

material

stainless steel column

agency

suitable for USP L43

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi)
60 °C temp. range

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 3 mm

surface area

120 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

PFP (pentafluorophenyl) phase

particle size

2 μm

pore size

90 Å pore size

operating pH

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

UHPLC/MS Analysis of Mycotoxins on Ascentis® Express F5, 2 μm

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

related product

Č. produktu
Popis
Stanovení ceny

14274

Acetonitrile with 0.1% ammonium acetate, tested for UHPLC-MS

1.03726

Methanol, for UHPLC-MS LiChrosolv®

1.03728

Water, for UHPLC-MS LiChrosolv®

1.03725

Acetonitrile, for UHPLC-MS LiChrosolv®

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
USFVE001327
USFVE001325
USFVE001324
USFVE001323
USFVE001322

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The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
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On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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