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Key Documents

S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Synonyma:

Saline-sodium citrate buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

form

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubility

water: 263 mg/mL, clear, colorless

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Features and Benefits

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Components

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Protokoly

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.