Přejít k obsahu
Merck
Všechny fotografie(1)

Key Documents

F9037

Sigma-Aldrich

Formamide

BioReagent, ≥99.5% (GC), for molecular biology

Synonyma:

Amide C1, Formic amide

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

Lineární vzorec:
HCONH2
Číslo CAS:
Molekulová hmotnost:
45.04
Beilstein/REAXYS Number:
505995
EC Number:
MDL number:
UNSPSC Code:
12352111
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

BioReagent

assay

≥99.5% (GC)

form

liquid

autoignition temp.

932 °F

technique(s)

electrophoresis: suitable
immunohistochemistry: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

suitability

suitable for nucleic acid hybridization

storage temp.

2-8°C

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

Hledáte podobné produkty? Navštivte Průvodce porovnáváním produktů

General description

Formamide is a clear liquid amide derived from formic acid. Formamide allows for the denaturation and renaturation of nucleic acids at room temperature.

Application

Formamide is suitable for use in hybridization buffers made using standard recipes. Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Other applications include:

  • Preparation of brain sections of mice for BrdU immunohistochemistry
  • FISH analysis of HeLa cells.
  • Preparation of the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis(The Merck Index, 12th ed).
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Features and Benefits

  • Deionized for molecular biology applications
  • Packaged under argon gas in amber bottles to protect from oxidation
  • Used for DNA renaturation or DNA-RNA hybridization
  • Reduces thermal stability of double stranded nucleic acids

Suitability

  • nucleic acid hybridization
  • cryoprotectant and gel-stabilizer
  • destabilize nucleic acid duplexes
  • elimination of secondary structure of nucleic acids
  • preparation of hybridization buffers for RNA and DNA application

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

target_organs

Blood

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

305.6 °F

flash_point_c

152 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

T Kaabache et al.
Analytical biochemistry, 232(2), 225-230 (1995-12-10)
The sensitivity of direct solution hybridization of hepatocytes solubilized in guanidium thiocyanate (GuSCN) for detecting alpha 1-acid glycoprotein and albumin mRNAs was studied. The sensitivity of detection was inversely correlated with the DNA concentration. Raising the hybridization temperature from 20
Laurent Geiser et al.
Journal of chromatography. A, 979(1-2), 389-398 (2002-12-25)
A nonaqueous capillary electrophoresis (NACE) method, coupled with either UV or electrospray mass spectrometry (ESI-MS), is described for the simultaneous analysis of seven beta-blockers. The same electrolyte, namely 25 mM ammonium formate and 1 M formic acid, was used with
N C Pagratis
Nucleic acids research, 24(18), 3645-3646 (1996-09-15)
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C
Jianwei Li
Journal of pharmaceutical sciences, 91(8), 1873-1879 (2002-07-13)
This article describes the proper selection of extraction solvents to eliminate interference from a polymer matrix to the quantitation of estradiol degradation products in a transdermal formulation by reversed-phase liquid chromatography. The separation is performed by gradient elution with acetonitrile
Helena Mistry et al.
Genome integrity, 1(1), 7-7 (2010-08-04)
DNA double-strand breaks (DSBs) caused by ionizing radiation or by the stalling of DNA replication forks are among the most deleterious forms of DNA damage. The ability of cells to recognize and repair DSBs requires post-translational modifications to histones and

Související obsah

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.