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Key Documents

D9905

Sigma-Aldrich

Denhardt′s Solution

for Northern and Southern blotting, lyophilized powder

Synonyma:

Denhardt′s Solution For Blotting Technique

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

form

lyophilized powder

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

2-8°C

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Denhardt′s Solution has been used:
  • as a component in hybridization buffer for the hybridization of brain sections
  • in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process
  • in the preparation of hybridization mixture

Suitable as a blocking Buffer for Southern and Northern blot membranes.

Biochem/physiol Actions

Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.

Components

Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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