PEROX1
Peroxisome Isolation Kit
isolate peroxisomes from tissues and cells
Synonyma:
Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Doporučené produkty
Quality Level
technique(s)
centrifugation: suitable
fractionation: suitable
shipped in
wet ice
Storage temp.
2-8°C
General description
Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.
Application
The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.
Features and Benefits
- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
- Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)
Other Notes
Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.
Legal Information
OptiPrep is a trademark of Serumwerk Bernburg AG
Součásti soupravy, které jsou dostupné také samostatně
Č. produktu
Popis
Bezpečností list
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionBezpečností list
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Zákazníci si také prohlíželi
P B Lazarow et al.
Proceedings of the National Academy of Sciences of the United States of America, 73(6), 2043-2046 (1976-06-01)
Purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-CoA oxidizing system that uses O2 and NAD as electron acceptors. The system was detected by the ability of added palmitoyl-CoA to elicit O2 consumption, H2O2 production, and O2-dependent NAD reduction. The
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
Dieanira Erudaitius et al.
Free radical biology & medicine, 120, 356-367 (2018-03-31)
The high extracellular hydrogen peroxide (H2O2) concentrations generated during pharmacological ascorbate (P-AscH-) therapy has been shown to exhibit a high flux into susceptible cancer cells leading to a decrease in clonogenic survival. It is hypothesized that the intracellular H2O2 concentration
The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol.
J Graham et al.
Analytical biochemistry, 220(2), 367-373 (1994-08-01)
This paper reports the use of a new density gradient compound, Iodixanol, for the resolution of the major organelles from mouse liver. A major advantage of Iodixanol over other iodinated density gradient media is its ready ability to form self-generated
Sortimentní položky
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.