The density of OptiPrep is expressed as weight/volume (w/v). The stock concentration from the bottle is 60%.
D1556
OptiPrep™ Density Gradient Medium
used for cell and subcellular organelle isolation
OptiPrep™ Density Gradient Medium
Synonyma:
Iodixanol solution
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 250 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 20 200,00 Kč |
O této položce
Číslo CAS:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352207
MDL number:
20 200,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohliQuality Level
sterility
sterile
form
liquid
density
1.319-1.321 g/mL
application(s)
hematology
histology
storage temp.
room temp
SMILES string
CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I
InChI
1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)
InChI key
NBQNWMBBSKPBAY-UHFFFAOYSA-N
General description
OptiPrep™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.[1]
Application
OptiPrep™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells.[1] It has also been used for the following applications:
- to investigate the efficiency of single-cell encapsulation.[1]
- the gradient-based isolation of senescent cancer cells.
- isolation of non-enveloped virion isolation from bronchoalveolar lavage.
- isolation of lipoproteins and macromolecules
- purification of virions
- purification of neuron cells
Features and Benefits
OptiPrep™ has numerous biocompatible properties that make it ideal for use.
- It is biologically inert and does not interfere with assays for marker enzymes.[1]
- It is non-ionic, non-toxic, and has low osmotic pressure.
- It forms spontaneous gradients that are stabler and easier to prepare than sucrose.
Other Notes
60% (w/v) solution of iodixanol in water (sterile).
Legal Information
OptiPrep is a trademark of Serumwerk Bernburg AG
1 of 1
Tato položka | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| density 1.319-1.321 g/mL | density - | density - | density - |
| sterility sterile | sterility - | sterility 0.2 μm filtered | sterility sterile-filtered |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. room temp | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
| application(s) hematology | application(s) - | application(s) sample preparation | application(s) - |
Skladovací třída
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Sortimentní položky
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome
Erica A. S, et al.
Emerging Infectious Diseases, 21(1), 103-106 (2015)
Tanner C Fadero et al.
eLife, 6 (2017-09-05)
Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
Isolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation
Kristina Kovacovicova, Manlio Vinciguerra
Cell Proliferation, 52 (2019)
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| D1556-100ML | 04061837280368 |
| D1556-250ML | 04061838261700 |
-
What is the density of OptiPrep: 60% v/v, w/w, or w/v?
1 answer-
Helpful?
-
-
What is the osmolarity of OptiPrep?
1 answer-
According to the supplier information, the osmolarity of OptiPrep is stated to be 170 mOsm with a deviation of +/- 15 mOsm.
Helpful?
-
-
What is the molarity of OptiPrep (catalog number D1556)?
1 answer-
The molecular weight of OptiPrep is 1552.2 amu, and its density is 1.320 g/ml. The prepared solution contains 60% OptiPrep. Based on these values, the calculated molarity is 0.706 Molar.
Helpful?
-
-
What is the method used to determine the density mentioned on the Certificate of Analysis (CoA) for D1556 OptiPrep™ Density Gradient Medium?
1 answer-
Based on the provided details, utilizing a densitometer or pycnometer is recommended.
Helpful?
-
-
What is the viscosity of the solution?
1 answer-
This product is not tested for viscosity. This item is a 60% solution of Iodixanol in water. However, Iodixanol properties reported by drugfuture.com state that a 50% solution has a viscosity of 8.6 mPa at 37°C.
Helpful?
-
-
How do I store solutions of Product D1556, OptiPrep™ Density Gradient Medium?
1 answer-
After diluting Product D1556, OptiPrep™ Density Gradient Medium, to the required density, the solution can be stored at 4°C. It should be mixed well before use to make sure it has not settled.
Helpful?
-
-
What is the appearance of Product D1556, OptiPrep?
1 answer-
The solution may be clear to slightly yellow.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Why choose Product D1556, OptiPrep, instead of other density gradients such as Percoll or Histopaque?
1 answer-
Percoll and Histopaque must be removed prior to use through washing. If Percoll and Histopaque are not removed, these density gradients can be toxic to mammalian cells. In comparison, OptiPrep has no potential for bioaccumulation and can be considered nontoxic to cells. OptiPrep also has lower viscosity as compared to polysucrose based products such as Histopaque or Ficoll.
Helpful?
-