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ISO2

Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Synonyma:

Mouse Antibody Isotyping Kit

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About This Item

MDL number:
MFCD08705607
UNSPSC Code:
12352203
NACRES:
NA.32

usage

sufficient for 1000 tests (clones) (by ELISA)
 sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

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General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

Pouze součásti soupravy

Č. produktu
Popis
  • goat antisera to mouse IgA .2 mL
  • goat antisera to mouse IgG3 .2 mL
  • goat antisera to mouse IgG1 .2 mL
  • goat antisera to mouse IgG2a .2 mL
  • goat antisera to mouse IgG2b .2 mL
  • goat antisera to mouse IgM .2 mL

related product

Č. produktu
Popis
Stanovení ceny

Storage Class

10 - Combustible liquids

Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number
0000218263
0000184924
088M4812V
037M4888V
046M4752V

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Potřebujete-li konkrétní verzi, můžete vyhledat daný certifikát podle čísla dávky nebo čísla šarže.

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Protokoly

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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