X4376

Xanthine Oxidase from bovine milk

lyophilized powder, 0.4-1.0 units/mg protein

• Synonyma: XOD, Xanthine:oxygen oxidoreductase
• Číslo CAS: 9002-17-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-657-6
• MDL number: MFCD00082145
• Číslo EC: 1.17.3.2.
• Specific activity: 0.4-1.0 units/mg protein
• Biological source: bovine milk

Vlastnosti

• biological source: bovine milk
• Quality Segment: 300
• form: lyophilized powder
• specific activity: 0.4-1.0 units/mg protein
• mol wt: 283 kDa
• composition: Protein, 10.0-25.0%
• storage temp.: 2-8°C

Popis

General description

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used as a source for superoxide generation. It has also been used as a model enzyme in the internally calibrated electrochemical continuous enzyme assay (ICECEA).

Biochem/physiol Actions

The isoflavanoid genistein was shown to inhibit xanthine oxidase at concentrations between 0.1-4.0 μM and then activate xanthine oxidase at concentrations higher than 5 μM.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Xanthine Oxidase acts as a potential biomarker for oxidative stress. It is also implicated in tissue and vascular injuries such as liver damage, inflammatory diseases and chronic heart failure.

Physical form

Contains 0.5% sodium salicylate.

Analysis Note

Protein determined by biuret

Other Notes

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)