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C8571

Sigma-Aldrich

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

Synonyma:

Cathepsin B1

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About This Item

Číslo CAS:
9047-22-7
Číslo enzymu podle klasifikace EK:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human liver

Quality Level

200

form

buffered aqueous solution

specific activity

≥1,500 units/mg protein (E1%/280)

UniProt accession no.

P07858

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CTSB(1508)

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:
  • as a positive control in the screening Cathepsin B inhibitor assay
  • in wall extension (expansin activity) assay
  • in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

inhibitor

Č. produktu
Popis
Stanovení ceny

E3132

E-64, protease inhibitor

M6159

α2-Macroglobulin from human plasma, BioUltra, ≥98% (SDS-PAGE)

L2023

Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial

A6191

Antipain dihydrochloride from microbial source, protease inhibitor

A6060

Calpain Inhibitor II, powder

A6185

Calpain Inhibitor I, ≥97% (TLC), powder

C7268

Chymostatin, microbial

related product

Č. produktu
Popis
Stanovení ceny

substrate

Č. produktu
Popis
Stanovení ceny

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number
0000336414
0000353058
0000328972
0000342604
0000302358

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Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways
Wu X, et al.
Hepatology, 47(6), 1905-1915 (2008)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
Yuanyuan Zhong et al.
Foods (Basel, Switzerland), 11(4) (2022-02-26)
This study aimed to evaluate the efficiency of microencapsulated apple polyphenols (MAP) in controlling cathepsin activity and texture, as well as inhibiting protein oxidation and metmyoglobin formation in lamb meat during frozen storage at -18 °C for 40 weeks. The
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Sortimentní položky

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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