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C7657

Sigma-Aldrich

Collagenase from Clostridium histolyticum

Type XI, 2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Synonyma:

Clostridiopeptidase A

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen

Vybrat velikost

25 MG
2 190,00 Kč
100 MG
4 640,00 Kč
500 MG
13 300,00 Kč
1 G
23 000,00 Kč
5 G
91 900,00 Kč

2 190,00 Kč


Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Vyžádat hromadnou objednávku

Vybrat velikost

Změnit zobrazení
25 MG
2 190,00 Kč
100 MG
4 640,00 Kč
500 MG
13 300,00 Kč
1 G
23 000,00 Kč
5 G
91 900,00 Kč

About This Item

Číslo CAS:
Číslo enzymu podle klasifikace EK:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

2 190,00 Kč


Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Vyžádat hromadnou objednávku

biological source

Clostridium histolyticum

Quality Level

form

powder

specific activity

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

mol wt

68-130 kDa

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Clostridiopeptidase A has been used in a study to describe a quantitative method to assess small amounts of collagen based on MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry (MS) subsequent to digestion of collagen.
This product has been used in the isolation of pancreatic ductal adenocarcinoma (PDAC) cell lines and the tissue culture of pancreatic fibroblasts.[1]

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of the reaction varies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn2+ is required for activity.

Quality

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Preparation Note

A stock solution may be prepared by dissolving 0.05-0.1 mg/mL of collagenase in 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4, at 37 °C.

substrate

Č. produktu
Popis
Stanovení ceny

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

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Eddie Rodríguez-Carballo et al.
Genes & development, 31(22), 2264-2281 (2017-12-24)
The mammalian
Maria M Glavas et al.
Scientific reports, 9(1), 3619-3619 (2019-03-08)
Childhood obesity and early rapid growth increase the risk for type 2 diabetes. Such early overnutrition can be modeled in mice by reducing litter size. We investigated the effects of early overnutrition and increased dietary fat intake on β cell
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Cellular signalling, 29, 41-51 (2016-11-05)
Recent evidence suggests that mammary cells expressing R-spondin receptor and Wnt pathway regulator Lgr5, regarded as a stem cell marker in multiple tissues, might represent mammary stem cells (MaSCs). Whether L gr5 marks a multipotent subpopulation of Lin-CD24low/medCD49fhigh MaSCs remains
Olivier Nolan-Stevaux et al.
Genes & development, 23(1), 24-36 (2009-01-13)
Pancreatic ductal adenocarcinoma (PDAC) is characterized by the deregulation of the hedgehog signaling pathway. The Sonic Hedgehog ligand (Shh), absent in the normal pancreas, is highly expressed in pancreatic tumors and is sufficient to induce neoplastic precursor lesions in mouse
Maria Molinos et al.
Spine, 43(5), E274-E284 (2017-07-06)
Human intervertebral disc (hIVD) cells were isolated from 41 surgically excised samples and assessed for their phenotypic alterations with age. Toward the design of novel anti-aging strategies to overcome degenerative disc disease (DDD), we investigated age-correlated phenotypic alterations that occur

Protokoly

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Questions

  1. How can I dissolve this product for Tissue digestion (Hanks Buffer or even medium possible)? Thanks

    1 answer
    1. This product is not tested specifically for use in cell culture, however, the product information provided for C9697 may be applied to this product. See the link below to review the product data-sheet. For tissue culture applications, collagenase can be solubilized in calcium-free solutions such as Hank's Balanced Salts (Cat. No. H 2387) or Earles Balanced Salt Solution (Cat. No. E6267).
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/390/021/c9697pis-ms.pdf

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